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Api listeria monocytogenes strip test

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  • 13.08.2018
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Abstract Listeria monocytogenes is responsible for causing the serious infectious disease listeriorosis which occurs due to food contaminated with Listeria. The study employed herein concerned with the isolation and identification of L. Hundred different food stuffs samples milk, cheese and meat and 50 fresh water samples were surveyed for presence of L. These samples were inoculated into two enrichment media and cultivated on Oxford agar.
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API Listeria, a new and promising one-day system to identify Listeria isolates.

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API for Microorganism Identification | bioMérieux

Listeria monocytogenes is responsible for causing the serious infectious disease listeriorosis which occurs due to food contaminated with Listeria. The study employed herein concerned with the isolation and identification of L. Hundred different food stuffs samples milk, cheese and meat and 50 fresh water samples were surveyed for presence of L. These samples were inoculated into two enrichment media and cultivated on Oxford agar. Presumptively positive isolates were determined by morphology of colonies, gram stain, catalase test, haemolysis on sheep blood agar and motility test. The isolates were further identified to species level by API Listeria strips, PCR was done for all specimens to evaluate its accuracy. Out of foodstuffs samples, 15 samples were showed positive results for Listria species.
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Discovered around years ago, it was a very occasional cause of meningitis and septicaemia, especially in newborn babies, but received little attention. All that began to change in , when it was first discovered that Listeria monocytogenes could be transmitted by contaminated food. Foodborne outbreaks of listeriosis were reported in North America and Europe in the s, and the incidence of the disease rose steadily year-by-year. These outbreaks led the World Health Organisation to conclude that L.
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API Listeria is a new test strip for h biochemical identification of Listeria isolates. A new test differentiates Listeria monocytogenes from L. With this system, Gram-positive bacteria other than Listeria spp.
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